LOCUS       B3GAT2-pGEn2            6207 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4835..5376
                     /note="WPRE"
     misc_recomb     4797..4819
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(5383..5597)
                     /note="BGH pA"
     misc_feature    complement(5685..5814)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..4790
                     /note="OPTIMIZED B3GAT2  coding region"
     vector          join(4805..6207,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..4804
                     /source="B3GAT2 pDONR"
                     /type="Entry Clone"
     source          1..6207
                     /dnas_title="B3GAT2-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc GACGTGGACA
     3901 CCAGACGGCC TGTGCCTCCA CTGACCCCTA GACCTTACTT TAGCCCTTAT GCCGTGGGCA
     3961 GAGGCGGAGC TAGACTGCCA CTGAGAAGAG GTGGACCTGC CCATGGCACC CAGAAGAGAA
     4021 ATCAGAGCAG ACCCCAGCCT CAGCCTGAGC CACAGCTGCC TACAATCTAC GCCATCACCC
     4081 CCACCTACTC CAGACCTGTG CAGAAGGCCG AACTGACCAG ACTGGCCAAC ACCTTTAGAC
     4141 AGGTGGCCCA GCTGCACTGG ATCCTGGTGG AAGATGCTGC CGCTAGAAGC GAGCTGGTGT
     4201 CTAGATTTCT GGCTAGAGCC GGCCTGCCTA GCACACATCT GCATGTGCCT ACCCCCAGAC
     4261 GGTACAAAAG ACCTGGACTG CCTAGAGCCA CCGAGCAGAG AAATGCTGGA CTGGCCTGGC
     4321 TGAGACAGAG GCATCAGCAT CAGAGAGCAC AGCCCGGCGT CCTGTTCTTC GCCGACGATG
     4381 ATAACACCTA CAGCCTGGAA CTGTTTCAGG AAATGCGGAC CACCCGGAAG GTGTCAGTGT
     4441 GGCCTGTGGG ACTCGTGGGC GGCAGAAGAT ACGAAAGACC TCTGGTCGAG AACGGCAAGG
     4501 TCGTGGGCTG GTATACAGGA TGGCGGGCTG ATAGACCCTT CGCCATCGAT ATGGCTGGCT
     4561 TCGCAGTCAG CCTGCAGGTC ATCCTGAGCA ACCCTAAGGC CGTGTTCAAG AGAAGGGGTA
     4621 GCCAGCCCGG AATGCAGGAA AGCGACTTCC TGAAGCAGAT CACCACCGTG GAAGAACTGG
     4681 AACCCAAGGC CAACAACTGC ACCAAAGTGC TCGTGTGGCA CACCCGGACC GAGAAAGTGA
     4741 ATCTGGCCAA TGAGCCCAAG TACCACCTGG ACACCGTGAA GATCGAGGTC TAGGACCCAG
     4801 CTTTCTTGTA CAAAGTGGTT GATAGCTTGG ATCCAATCAA CCTCTGGATT ACAAAATTTG
     4861 TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC
     4921 TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA
     4981 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT
     5041 GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA
     5101 GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC
     5161 CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT
     5221 GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG
     5281 CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG
     5341 CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGAGAT CTGCCTCGAC TGTGCCTTCT
     5401 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC
     5461 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT
     5521 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT
     5581 AGCAGGCATG CTGGGGACTC GAGTTAAGGG CGAATTGCCG ATTAGGATCT TCCTAGAGCA
     5641 TGGCTACGTA GATAAGTAGC ATGGCGGGTT AATCATTAAC TACAAGGAAC CCCTAGTGAT
     5701 GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT
     5761 CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCCTTAA
     5821 TTAAGAGCTC ATGGCGCGCC TAGGCCTTGA CGGCCTTCCG CCAATTCGCC CTATAGTGAG
     5881 TCGTATTACG TCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
     5941 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
     6001 AGAGGCCCGC ACCGAAACGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGAGC
     6061 GCCCTGTAGC GGCCACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
     6121 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
     6181 AACAAAATAT TAACGCTTAC AATTTAG
//

